Optical microscope bright field
The bright field exposure, which is the result of placing a dark object on a dark background, is the simplest optical microscopic technique. In the bright field exposure, the light source is placed below the sample. Then the light passes through the sample and is observed by the objective lens (objective) and the sensor located above the sample. The simplicity of the clear background method is the main reason for the popularity of this microscopic method.
Common images The bright background microscopes include a dark sample in a bright background. The more points on the sample are darker, the more light they will be absorbed. For example, plant cells in the nucleus and central regions where the cellular materials are denser appear darker and appear more rigid in the cytoplasm of the ribosome, the endoplasmic network, and other intracellular components. Animal cells without coloring, which ultimately lead to the death of living cells, are hardly imaging by this method.
Optical microscope bright background | INFORTEX COMPANY
Figure 1) Illuminated background light on the tissue paper
In the optical path, there is a clear field microscope with four key components:
Optical source: Usually a broadband optical source such as halogen quartz lamp is used for exposure to the sub-specimen.
Density Lens: Focuses light from an optical source on the sample.
Object Lens: Collect light from the sample and increase the visibility of detail by magnifying.
Eye / Camera: View or capture an image
The limitations of this microscopic method include very poor image contrast for cellular or biological samples, low optical resolution due to optical constraints and the need for colored specimens before viewing or imaging. Although the simplicity of this technique is a great benefit for the first observation of anonymous samples.